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Western blot analysis of extracts from HCC827 cells, mock transfected or transfected with GFP, using GFP (D5.1) XP® Rabbit mAb (HRP Conjugate).

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

GFP (D5.1) XP® Rabbit mAb (HRP Conjugate) detects GFP-tagged proteins exogenously expressed in cells.

Species Reactivity: All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP. This antibody was conjugated to HRP under optimal conditions.

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).

1.  Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.