Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee



Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.


Endogenous 80 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

This antibody detects endogenous levels of Rev-erbα protein. The antibody does not cross-react with other nuclear receptor proteins, including Rev-erbβ.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human Rev-erbα protein. Antibodies are purified by protein A and peptide affinity chromatography.

Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3-7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10).

1.  Harding, H.P. and Lazar, M.A. (1993) Mol Cell Biol 13, 3113-21.

2.  Chawla, A. and Lazar, M.A. (1993) J Biol Chem 268, 16265-9.

3.  Torra, I.P. et al. (2000) Endocrinology 141, 3799-806.

4.  Preitner, N. et al. (2002) Cell 110, 251-60.

5.  Vu-Dac, N. et al. (1998) J Biol Chem 273, 25713-20.

6.  Coste, H. and Rodríguez, J.C. (2002) J Biol Chem 277, 27120-9.

7.  Raspé, E. et al. (2002) J Lipid Res 43, 2172-9.

8.  Yin, L. et al. (2006) Science 311, 1002-5.

9.  Migita, H. et al. (2004) FEBS Lett 561, 69-74.

10.  Yin, L. and Lazar, M.A. (2005) Mol Endocrinol 19, 1452-9.

Entrez-Gene Id 9572
Swiss-Prot Acc. P20393

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.