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PTMScan® Motif Antibody Kits
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Phospho-(Thr) MAPK/CDK Substrate Mouse mAb #2321
This product is discontinued
Gallery: Phospho-(Thr) MAPK/CDK Substrate Mouse mAb #2321
Phospho-(Thr) MAPK/CDK Substrate Mouse mAb detects phospho-threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody does not cross-react with phospho-threonine in the absence of an adjacent proline. The antibody does not cross-react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides . This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody. Antibody is purified by protein A chromatography.
The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline.
As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-(Thr) MAPK/CDK Substrate Monoclonal Antibody recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by Western blot analysis of nocodazole-treated Jurkat cell extracts resolved on a 2-D gel.
Product Specific References
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at email@example.com.