News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.


PTMScan® Motif Antibody Kits
New Format-Same Price

Now 10 Assays Per Kit

Order Now


Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.


We recommend the following alternatives

W   IHC       H M R All

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-(Thr) MAPK/CDK Substrate Mouse mAb detects phospho-threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody does not cross-react with phospho-threonine in the absence of an adjacent proline. The antibody does not cross-react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides . This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody. Antibody is purified by protein A chromatography.

The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To facilitate the study and discovery of new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine or phospho-serine followed by proline.

As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-(Thr) MAPK/CDK Substrate Monoclonal Antibody recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by Western blot analysis of nocodazole-treated Jurkat cell extracts resolved on a 2-D gel.

1.  Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.

2.  Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.

3.  Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.

4.  Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.

5.  Nurse, P. (2000) Cell 100, 71-8.

6.  Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at For information regarding commercial licensing terms please contact CST Pharma Services Department at

Phospho-(Thr) MAPK/CDK Substrate Mouse mAb