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MBP Tag (8G1) Mouse mAb #2396
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Gallery: MBP Tag (8G1) Mouse mAb #2396
MBP Tag (8G1) Mouse mAb detects recombinant proteins fused to maltose binding protein (MBP).Species Reactivity: All Species Expected
Monoclonal antibody is produced by immunizing animals with an MBP fusion protein.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
One commonly used system is the maltose-binding protein (MBP) (1). In this system, the target gene is inserted in the appropriate vector and expressed. Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein (2). An antibody to the MBP tag can also be used to isolate or detect expression of the protein.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.