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REACTIVITY SENSITIVITY MW (kDa) SOURCE
Endogenous 62 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Chk2 (Thr387) Antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 387. The antibody does not cross-react with Chk2 phosphorylated at other sites.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr387 of human Chk2. Antibodies are purified by protein A and peptide affinity chromatography.

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).


1.  Ahn JY et al. (2000) Cancer Res 60, 5934–6

2.  Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.

3.  Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.

4.  Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.

5.  Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.

6.  Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.

7.  Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.

8.  Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.


Entrez-Gene Id 11200
Swiss-Prot Acc. O96017


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