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MW (kDa)
110 kDa recombinant kinase fragment

Western blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), untreated or λ phosphatase-treated, using Phospho-VEGF Receptor 2 (Tyr1175) Antibody #2478 (upper), VEGF Receptor 2 Antibody #2479 (lower).

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Product Description

Nonphosporylated VEGFR2 Control Proteins: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells treated with λ phosphatase. Supplied in SDS Sample Buffer. Store at -20ºC.

Phosphorylated VEGFR2 Control Proteins: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells. Supplied in SDS Sample Buffer. Store at -20ºC.


Product Usage Information

As controls, we recommend using 15 µl (20 ng) of phosphorylated and nonphosphorylated VEGFR2 control proteins. Boil sample before use.


Storage: Supplied in SDS Sample Buffer: 62.5 mM Tris-HCL (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C.

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).


1.  Meyer, M. et al. (1999) EMBO J 18, 363-74.

2.  Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.

3.  Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.

4.  Takahashi, T. et al. (2001) EMBO J 20, 2768-78.

5.  Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.

6.  Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.

7.  Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.

8.  Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.


Entrez-Gene Id 3791
Swiss-Prot Acc. P35968


For Research Use Only. Not For Use In Diagnostic Procedures.
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