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140 Rabbit

Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MYPT1 (Ser668) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Ser668.

Species Reactivity: Human
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser668 of human MYPT1. Antibodies are purified using protein A and peptide affinity chromatography.

Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1).

The myosin phosphatase holoenzyme is composed of three subunits: PP1c, a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase), and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed, while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members include MBS85, MYPT3, and TIMAP (4).

Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6).

Phospho-MYPT1 (Ser668) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser668 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at for more information.

1.  Cohen, P.T. (2002) J Cell Sci 115, 241-56.

2.  Terrak, M. et al. (2004) Nature 429, 780-4.

3.  Fujioka, M. et al. (1998) Genomics 49, 59-68.

4.  Ito, M. et al. (2004) Mol Cell Biochem 259, 197-209.

5.  Birukova, A.A. et al. (2004) Microvasc Res 67, 64-77.

6.  Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

Entrez-Gene Id 4659
Swiss-Prot Acc. O14974

For Research Use Only. Not For Use In Diagnostic Procedures.
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