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H M R Mk Endogenous 49 Rabbit

Western blot analysis of extracts from various cell lines and tissues using Flotillin-1 Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Flotillin-1 Antibody detects endogenous levels of total flotillin-1 protein.

Species Reactivity: Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Ile368 of human flotillin-1. Antibodies are purified by peptide affinity chromatography.

Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

1.  Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.

2.  Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.

3.  Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.

4.  Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.

5.  Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.

6.  Liu, J. et al. (2005) J Biol Chem 280, 16125-34.

7.  Santamaría, A. et al. (2005) Mol Cell Biol 25, 1900-11.

8.  Solomon, S. et al. (2002) Immunobiology 205, 108-19.

9.  Neumann-Giesen, C. et al. (2004) Biochem J 378, 509-18.

Entrez-Gene Id 10211
Swiss-Prot Acc. O75955

For Research Use Only. Not For Use In Diagnostic Procedures.
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