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REACTIVITY SENSITIVITY MW (kDa)
60

Western blot analysis of extracts from SR, Hel and THP-1 cell lines using IRF-5 Antibody.

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Confocal immunofluorescent analysis of THP-1 cells using IRF-5 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).

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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

IRF-5 Antibody detects endogenous levels of total IRF-5 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human IRF-5 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).


IRF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection (3). Activation of IRF-5 signaling is triggered by the toll-like receptor (TLR) pathway, including TLR7 and MyD88 (4,5). Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus (6,7).


1.  Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.

2.  Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.

3.  Barnes, B.J. et al. (2001) J Biol Chem 276, 23382-90.

4.  Takaoka, A. et al. (2005) Nature 434, 243-9.

5.  Schoenemeyer, A. et al. (2005) J Biol Chem 280, 17005-12.

6.  Sigurdsson, S. et al. (2005) Am J Hum Genet 76, 528-37.

7.  Graham, R.R. et al. (2006) Nat Genet 38, 550-5.


Entrez-Gene Id 3663
Swiss-Prot Acc. Q13568


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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DRAQ5® is a registered trademark of Biostatus Limited.