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40 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Rad52 Antibody detects endogenous levels of total Rad52 protein.

Species predicted to react based on 100% sequence homology: Hamster

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human Rad52. Antibodies are purified by protein A and peptide affinity chromatography.

Repair of DNA double-stranded breaks (DSBs) can occur via nonhomologous end joining (NHEJ), homologous recombination (HR), or other DSB repair pathways (1). In yeast, one family of proteins involved in HR is the Rad52 group, which includes Rad52 itself, Rad51, and Rad54 (1). Vertebrate cells lacking Rad51, Rad52, or Rad54 often show enhanced sensitivity to ionizing radiation (IR) or chromosomal abnormatlities (2-4). In response to IR-induced DNA damage, ATM activates the kinase c-Abl, which phosphorylates Rad51 at Tyr54 and Tyr315, and Rad52 at Tyr104, causing translocation of these proteins to sites of DNA strand breaks/subnuclear foci (5-7).

1.  Paques, F. and Haber, J.E. (1999) Microbiol. Mol. Biol. Rev. 63, 349-404.

2.  Bezzubova, O. et al. (1997) Cell 89, 185-193.

3.  Takata, M. et al. (2000) Mol. Cell. Biol. 20, 6476-6482.

4.  Fujimori, A. et al. (2001) EMBO J. 20, 5513-5520.

5.  Chen, G. et al. (1999) J. Biol. Chem. 274, 12748-12752.

6.  Yuan, Z. M. et al. (1998) J. Biol. Chem. 273, 3799-3802.

7.  Kitao, H. and Yuan, Z.M. (2002) J. Biol. Chem. 277, 48944-48948.

Entrez-Gene Id 5893
Swiss-Prot Acc. P43351

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Rad52 Antibody