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To Purchase # 3655S

3655S 100 µl (100 tests) $299.00
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REACTIVITY SENSITIVITY Isotype
H M R Endogenous Mouse IgG1

Confocal immunofluorescent analysis of rat brain using GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Usage Information

Application Dilutions
Immunofluorescence 1:100

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total GFAP protein.


Species Reactivity: Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescence of rat cells. The unconjugated antibody GFAP (GA5) Mouse mAb #3670 reacts with human, mouse and rat GFAP protein. CST expects that GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) will also recognize GFAP in these species.


The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3).


1.  Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.

2.  Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.

3.  Jessen, K.R. et al. (1990) Development 109, 91-103.


Entrez-Gene Id 2670
Swiss-Prot Acc. P14136


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.