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Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677
Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 1X SDS loading buffer with DTT using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb (Panel B), or Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected by Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.Learn more about how we got this image
Gallery: Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677
Affinity purified mouse anti-rabbit IgG (Light-Chain Specific) antibody. This product has been optimized for use as a secondary antibody in Western blotting applications.
Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb recognizes the light chain of rabbit IgG, either in native conformation or denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb as a secondary antibody will eliminate this problem.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.