News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
CST Antibody Performance Guarantee
To Purchase # 3678S
|3678S||100 µl (20 western blots)||$139.00|
|$ 0. 00|
Find answers on our FAQs page.
PTM information and tools available.
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678
Western blot analysis of decreasing concentrations of total rabbit IgG, reduced and denatured in 1X SDS loading buffer with DTT, using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 (Panel B), or Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected using Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.Learn more about how we got this image
Gallery: Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678
Affinity purified mouse anti-rabbit IgG (Conformation Specific) antibody. This product has been optimized for use as a secondary antibody in Western blotting applications.
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb recognizes the native rabbit IgG. It does not recognize the denatured and reduced rabbit IgG heavy (about 50 kDa) or light (about 25 kDa) chains on western blot.
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.