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REACTIVITY SENSITIVITY MW (kDa) SOURCE
70-80 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TGF-β Receptor II (K105) Antibody detects endogenous levels of total TGF-β receptor II protein.


Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys105 (extracellular region) of human TGF-β receptor II. Antibodies were purified by protein A and peptide affinity chromatography.

Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).


1.  Massague, J. et al. (2000) Cell 103, 295-309.

2.  Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.

3.  Derynck, R. et al. (2001) Nature Genet. 29, 117-129.

4.  Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.

5.  Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.

6.  López-Casillas, F. et al. (1991) Cell 67, 785-795.

7.  Wang, X.F. et al. (1991) Cell 67, 797-805.

8.  Derynck, R. et al. (1998) Cell 95, 737-740.

9.  Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.


Entrez-Gene Id 7048
Swiss-Prot Acc. P37173


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

3713
TGF-β Receptor II (K105) Antibody