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SENSITIVITY Isotype
Mouse 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

GST (26H1) Mouse mAb (Alexa Fluor® 555 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST fusion protein. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio 2-6.

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the biochemical properties of tagged proteins.


Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).


1.  Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.

2.  Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.

3.  Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.