Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY SENSITIVITY MW (kDa) SOURCE
18 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Pin1 (Ser16) Antibody detects endogenous levels of Pin1 only when phosphorylated at serine 16.


Species predicted to react based on 100% sequence homology: Human, Mouse, Xenopus

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser16 of human Pin1. Antibodies are purified by protein A and peptide affinity chromatography.

Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).


Phosphorylation of pin1 at Ser16 affects its subcellular localization and the ability of its WW domain to interact with potential targets (10).


1.  Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643.

2.  Lu, K. P. et al. (1996) Nature 380, 544-547.

3.  Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883.

4.  Wu, X. et al. (2000) EMBO J. 19, 3727-3738.

5.  Winkler, K. E. et al. (2000) Science 287, 1644-1647.

6.  Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327.

7.  Shen, M. et al. (1998) Genes Dev. 12, 706-720.

8.  Yaffe, M.B. et al. (1997) Science 278, 1957-60.

9.  Lu, P.J. et al. (1999) Science 283, 1325-8.

10.  Lu, P. J. et al. (2002) J Biol Chem 277, 2381-2384.


Entrez-Gene Id 5300
Swiss-Prot Acc. Q13526


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.