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REACTIVITY SENSITIVITY MW (kDa) Isotype
H Endogenous Rabbit IgG

Validation of Phospho-Ezrin (Thr567)/ Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb (ELISA Specific) in peptide DELFIA® assay using phospho- and nonphospho-peptide controls, and DELFIA® secondary antibodies (available from Perkin Elmer Life and Analytical Sciences).

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Product Usage Information

Application Dilutions
Peptide ELISA 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb (ELISA Specific) is phospho-specific by Peptide-ELISA and exhibits a large signal to noise window. The antibody does not recognize the non-phosphorylated peptide in peptide based ELISA (see figure below).


Species Reactivity: Human
Species predicted to react based on 100% sequence homology: Mouse, Rat, Bovine

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein.

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).


1.  Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10.

2.  Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92.

3.  Matsui, T. et al. (1998) J Cell Biol 140, 647-57.

4.  Gautreau, A. et al. (2000) J Cell Biol 150, 193-203.

5.  Tran Quang, C. et al. (2000) EMBO J 19, 4565-76.

6.  Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5.


Entrez-Gene Id 7430, 4478, 5962
Swiss-Prot Acc. P15311, P26038, P35241


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.
U.S. Patent No. 5,675,063.