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Western blot analysis of extracts from SH-SY5Y cells, forskolin-treated (30 µM for 20 min.) or untreated, using Phospho-CREB (Ser133) Antibody #9191 (upper) or CREB Antibody #9192 (lower).

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Product Usage Information

Forskolin is soluble in DMSO and methanol. DMSO solutions can be stored at -20ºC in the dark for at least six months.


Storage: Store lyophilized or in solution at -20°C. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency.

Product Description

Molecular Weight:

410.5 g/mol


Purity:

>99%


Molecular Formula:

C22H34O7


Forskolin, a naturally occurring diterpene from the Indian plant, Coleus forskohlii, activates adenylate cyclase, and thus increases the intracellular cAMP concentration (1). The second messenger cAMP activates cAMP-dependent protein kinase (PKA or cAPK) and controls many cellular mechanisms such as gene transcription, ion transport and protein phosphorylation (2).


Purity is greater than 99% as determined by HPLC and TLC.


1.  Laurenza, A. et al. (1989) Trends Pharmacol Sci 10, 442-7.

2.  Montminy, M. (1997) Annu Rev Biochem 66, 807-22.



For Research Use Only. Not For Use In Diagnostic Procedures.
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