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H M R Endogenous 32 , 35, 36, 41 Rabbit IgG

Western blot analysis of extracts from human, mouse and rat heart tissue and C2C12 cells using Tropomyosin-1/3 (D17B8) Rabbit mAb.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Tropomyosin-1/3 (D17B8) Rabbit mAb detects endogenous levels of total tropomyosin-1 and/or tropomyosin-3 protein.

Species Reactivity: Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu274 of human tropomyosin-1 protein.

Tropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1,2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1,2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10).

1.  Perry, S.V. (2001) J Muscle Res Cell Motil 22, 5-49.

2.  Lin, J.J. et al. (2008) Adv Exp Med Biol 644, 201-22.

3.  Brown, J.H. et al. (2001) Proc Natl Acad Sci USA 98, 8496-501.

4.  Rajan, S. et al. (2007) Circ Res 101, 205-14.

5.  Bharadwaj, S. et al. (2004) J Biol Chem 279, 14039-48.

6.  Mahadev, K. et al. (2002) Exp Cell Res 279, 40-51.

7.  Varga, A.E. et al. (2005) Oncogene 24, 5043-52.

8.  Raval, G.N. et al. (2003) Oncogene 22, 6194-203.

9.  Houle, F. et al. (2003) Mol Biol Cell 14, 1418-32.

10.  Houle, F. et al. (2007) J Cell Sci 120, 3666-77.

Entrez-Gene Id 7168
Swiss-Prot Acc. P09493

For Research Use Only. Not For Use In Diagnostic Procedures.
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