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Immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green), β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red) and DAPI (blue).Learn more about how we got this image
Gallery: DAPI #4083
DAPI is supplied as a lyophilized powder in 1 mg units. It can be used to examine cellular DNA in fluorescent microscopy applications.Molecular Formula:
- Free dye excitation maximum = 340 nm
- Free dye emission maximum = 488 nm
- DNA complex excitation maximum = 364 nm
- DNA complex emission maximum = 454 nm
This product is soluble up to 20 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, DAPI stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 to 0.1 μg/mL.Storage: Store desiccated and protected from light at 2-8°C. Lyophilized DAPI is stable for 2 years under these conditions. Soluble DAPI is stable for at least 2-3 weeks if protected from light.
4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.