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135 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MDA-5 (R470) Antibody detects endogenous levels of total MDA-5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrouding Arg470 of human MDA-5. Antibody is purified by protein A and peptide affinity chromatography.

Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).

MDA-5 (16,17), also named Ifih1 (interferon induced with helicase C domain 1), RH116 (RNA helicase-DEAD box protein 116) (18), and Helicard (19) is found to be induced by interferon. During apoptosis, MDA-5 is cleaved by caspases, separating the helicase and CARD domains (19). MDA-5 is uniquely activated by picornavirus (20) and measles virus (21).

1.  Yoneyama, M. and Fujita, T. (2007) J Biol Chem 282, 15315-8.

2.  Seth, R.B. et al. (2005) Cell 122, 669-682.

3.  Meylan, E. and Tschopp, J. (2006) Mol Cell 22, 561-9.

4.  Thompson, A.J. and Locarnini, S.A. (2007) Immunol Cell Biol 85, 435-45.

5.  Xu, L.G. et al. (2005) Mol. Cell 19, 727-740.

6.  Imaizumi, T. et al. (2002) Biochem Biophys Res Commun 292, 274-9.

7.  Yoneyama, M. et al. (2004) Nat. Immunol. 5, 730-737.

8.  Zhang, X. et al. (2000) Microb Pathog 28, 267-78.

9.  Yoneyama, M. et al. (2005) J Immunol 175, 2851-8.

10.  Hornung, V. et al. (2006) Science 314, 994-7.

11.  Pichlmair, A. et al. (2006) Science 314, 997-1001.

12.  Kato, H. et al. (2006) Nature 441, 101-5.

13.  Childs, K. et al. (2007) Virology 359, 190-200.

14.  Meylan, E. et al. (2005) Nature 437, 1167-72.

15.  Kawai, T. et al. (2005) Nat Immunol 6, 981-8.

16.  Kato, H. et al. (2006) Nature 441, 101-5.

17.  Kang, D.C. et al. (2002) Proc Natl Acad Sci U S A 99, 637-42.

18.  Kang, D.C. et al. (2004) Oncogene 23, 1789-800.

19.  Cocude, C. et al. (2003) J Gen Virol 84, 3215-25.

20.  Kovacsovics, M. et al. (2002) Curr Biol 12, 838-43.

21.  Berghäll, H. et al. (2006) Microbes Infect 8, 2138-44.

Entrez-Gene Id 64135
Swiss-Prot Acc. Q9BYX4

For Research Use Only. Not For Use In Diagnostic Procedures.
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MDA-5 (R470) Antibody