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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TMS1 Antibody detects endogenous levels of total TMS1 protein.

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse TMS1. Antibodies are purified by protein A and peptide affinity chromatography.

TMS1 (target of methylation-induced silencing)/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of TMS1 can be induced by pro-apoptotic/inflammatory stimuli (9). During apoptosis TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).

1.  Masumoto, J. et al. (1999) J Biol Chem 274, 33835-8.

2.  Conway, K.E. et al. (2000) Cancer Res 60, 6236-42.

3.  Terasawa, K. et al. (2004) Clin Cancer Res 10, 2000-6.

4.  Stone, A.R. et al. (2004) Am J Pathol 165, 1151-61.

5.  Guan, X. et al. (2003) Int J Cancer 107, 202-8.

6.  Moriai, R. et al. (2002) Anticancer Res 22, 4163-8.

7.  Virmani, A. et al. (2003) Int J Cancer 106, 198-204.

8.  Das, P.M. et al. (2006) Mol Cancer 5, 28.

9.  Strong, R. et al. (1991) Brain Res 542, 23-8.

10.  Ohtsuka, T. et al. (2004) Nat Cell Biol 6, 121-8.

11.  Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.

Entrez-Gene Id 29108
Swiss-Prot Acc. Q9ULZ3

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

TMS1 Antibody