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4878S 100 µl $109.00
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REACTIVITY SENSITIVITY Isotype
Endogenous Mouse IgG1

Flow cytometric analysis of untreated Jurkat cells using Mouse (MOPC-21) mAb IgG1 Isotype control (Alexa Fluor® 488 Conjugate) (red) compared to Akt (5G3) Mouse mAb (Alexa Fluor® 488 Conjugate) #2917(green).

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Flow cytometric analysis of U0126-treated Jurkat cells using Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (Alexa Fluor® 488 Conjugate) #4374, U0126-treated (blue) or PMA-treated (green).

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Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

The antibody is not directed against any known antigen. It functions as an isotype control for mouse monoclonal antibodies. This antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.


Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis of human cells.


Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.