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21 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Rheb Antibody detects endogenous levels of Rheb. The antibody does not cross-react with other family members at physiological levels.

Species predicted to react based on 100% sequence homology: Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn50 of Rheb. Antibodies are purified by protein A and peptide affinity chromatography.

Ras Homolog Enriched in Brain (Rheb) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other Ras family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb through the tuberin GAP activity (7).

1.  Yamagata, K. et al. (1994) J Biol Chem 269, 16333-9.

2.  Yee, W.M. and Worley, P.F. (1997) Mol Cell Biol 17, 921-33.

3.  Inoki, K. et al. (2003) Genes Dev 17, 1829-34.

4.  Stocker, H. et al. (2003) Nat Cell Biol 5, 559-65.

5.  Saucedo, L.J. et al. (2003) Nat Cell Biol 5, 566-71.

6.  Zhang, Y. et al. (2003) Nat Cell Biol 5, 578-81.

7.  Li, Y. et al. (2004) Trends Biochem Sci 29, 32-8.

Entrez-Gene Id 6009
Swiss-Prot Acc. Q15382

Product Specific References

Sancak, Y. et al. (2010) Cell 141, 290-303.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Rheb Antibody