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REACTIVITY SENSITIVITY MW (kDa) Isotype
51 Mouse 

DELIFA® data generated using biotinylated phospho- and nonphospho-peptides. Protocols and S/N values available upon request.

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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-GSK-3alpha (Ser21) (27E5) Monoclonal Antibody can be used in high throughput kinase assays and drug discovery applications. It detects peptides derived from GSK-3alpha phosphorylated at Ser21.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3alpha. Antibody is purified using protein G affinity chromatography.

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).


1.  Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.

2.  Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.

3.  Nusse, R. (1997) Cell 89, 321-323.

4.  Cross, D.A. et al. (1995) Nature 378, 785-9.

5.  Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.


Entrez-Gene Id 2931
Swiss-Prot Acc. P49840


For Research Use Only. Not For Use In Diagnostic Procedures.
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