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To Purchase # 5217SC

5217SC 10 µg (With Carrier) $199.00
5217SF 10 µg (Carrier Free) $199.00
5217LC 50 µg (With Carrier) $599.00
5217LF 50 µg (Carrier Free) $599.00
$ 0. 00

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Source / Purification

Recombinant mouse IL-7 (mIL-7) Glu26-Ile154 (Accession #NP_032397) was produced in E. coli at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-7. All lots are greater than 98% pure.


Molecular Formula:

Recombinant mIL-7 has a Met on the amino terminus and has a calculated MW of 15,028. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal MECHI of recombinant mIL-7 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 60-200 pg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg mIL-7.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory αβ T cells, as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7-specific IL-7Rα (1). IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5 (1).


1.  Rochman, Y. et al. (2009) Nat Rev Immunol 9, 480-90.

2.  Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.

3.  Parrish, Y.K. et al. (2009) J Immunol 182, 4255-66.


Entrez-Gene Id 16196
Swiss-Prot Acc. P10168


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.