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To Purchase # 5223SC

5223SC 10 µg (With Carrier) $199.00
5223SF 10 µg (Carrier Free) $199.00
5223LC 50 µg (With Carrier) $599.00
5223LF 50 µg (Carrier Free) $599.00
$ 0. 00

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Source / Purification

Recombinant mouse SCF (mSCF) Lys26-Ala189 (Accession #NP_038626) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mSCF. All lots are greater than 98% pure.


Molecular Formula:

Recombinant mSCF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,298. DTT-reduced and non-reduced protein migrate as 22-35 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal KEICG of recombinant mSCF was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mSCF was determined in an M-07e cell proliferation assay. The ED50 of each lot is between 2-8 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg mSCF.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mSCF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6).


1.  Broudy, V.C. (1997) Blood 90, 1345-64.

2.  Huang, B. et al. (2008) Blood 112, 1269-79.

3.  Yasuda, A. et al. (2006) Mol Cancer 5, 46.

4.  Pan, P.Y. et al. (2008) Blood 111, 219-28.

5.  Samayawardhena, L.A. and Pallen, C.J. (2008) J Biol Chem 283, 29175-85.

6.  Huang, H.M. et al. (2000) Blood 96, 1764-71.


Entrez-Gene Id 17311
Swiss-Prot Acc. P20826


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.