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To Purchase # 5314SC

5314SC 10 µg (With Carrier) $199.00
5314SF 10 µg (Carrier Free) $199.00
5314LC 50 µg (With Carrier) $599.00
5314LF 50 µg (Carrier Free) $599.00
$ 0. 00

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Source / Purification

Recombinant mouse VEGF120 (mVEGF120) Ala27-Arg146 (Accession #NP_001020428) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF120. All lots are greater than 98% pure.


Molecular Formula:

Recombinant mVEGF120 contains no "tags" and the nonglycosylated protein has a calculated MW of 14,071. DTT-reduced protein migrates as a 20-26 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 32-37 kDa protein. The expected amino-terminal APTTE of recombinant hVEGF120 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mVEGF120 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-5 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1μg mVEGF120.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mVEGF120. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

VEGF120 , a splice variant of the VEGF-A gene, is the murine counterpart to human VEGF121 (1,2). VEGF120 is produced by endothelial cells, macrophages, T cells, and others. VEGF120 is involved in angiogenesis, vascular permeability, vascular endothelial cell survival, growth, and migration (1). VEGF120 expression is induced by hypoxia, inflammatory cytokines, and through oncogene products in tumors (1,2). VEGF120 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF120 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/Akt, p38, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).


1.  Takahashi, H. and Shibuya, M. (2005) Clin Sci (Lond) 109, 227-41.

2.  Neufeld, G. et al. (1999) FASEB J 13, 9-22.


Entrez-Gene Id 22339
Swiss-Prot Acc. Q00731


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.