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To Purchase # 5367SC

5367SC 10 µg (With Carrier) $199.00
5367SF 10 µg (Carrier Free) $199.00
5367LC 50 µg (With Carrier) $599.00
5367LF 50 µg (Carrier Free) $599.00
$ 0. 00

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Source / Purification

Recombinant human Oncostatin M (hOSM) Ala26-Arg221 (Accession #NP_065391) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hOSM. All lots are greater than 98% pure.


Molecular Formula:

Based on amino acid sequencing, greater than 85% of recombinant hOSM starts at Aa26 (AAIGS) and has a calculated MW of 19,249. The remainder starts at Ile28 (IGSCS). DTT-reduced and non-reduced protein migrate as 32 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.


Bioactivity:

The bioactivity of recombinant hOSM was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 50-500 pg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hOSM.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hOSM. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. In contrast, Mouse OSM binds to the OSM receptor β (OSMRβ) which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). OSM induces the activation the Jak2, Stat3, and Erk1/2 pathways (5).


1.  Malik, N. et al. (1989) Mol Cell Biol 9, 2847-53.

2.  Silver, J.S. and Hunter, C.A. (2010) J Leukoc Biol 88, 1145-56.

3.  Underhill-Day, N. and Heath, J.K. (2006) Cancer Res 66, 10891-901.

4.  Ichihara, M. et al. (1997) Blood 90, 165-73.

5.  Hintzen, C. et al. (2008) J Immunol 181, 7341-9.


Entrez-Gene Id 5008
Swiss-Prot Acc. P13725


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.