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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Tau (Thr181) Antibody recognizes endogenous levels of Tau protein only when phosphorylated at Thr181.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr181 of human Tau protein. Antibodies are purified by protein A and peptide affinity chromatography.

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

The cerebrospinal fluid concentration of Tau phosphorylated at Thr181 has been proposed to be a biomarker for the study of neurodegenerative disorders (4).

1.  Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.

2.  Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.

3.  Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

4.  Mitchell, A.J. (2009) J Neurol Neurosurg Psychiatry 80, 966-75.

Entrez-Gene Id 4137
Swiss-Prot Acc. P10636-8

For Research Use Only. Not For Use In Diagnostic Procedures.
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Phospho-Tau (Thr181) Antibody