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32 Rabbit 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb detects endogenous levels of DARPP-32 only when phosphorylated at Thr34. This antibody may also cross-react with an unidentified 40 kDa protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr34 of human DARPP-32.

DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).

1.  Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.

2.  Bibb, J.A. et al. (1999) Nature 402, 669-671.

3.  Fienberg, A.A. et al. (1998) Science 281, 838-842.

Entrez-Gene Id 84152
Swiss-Prot Acc. Q9UD71

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb