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To Purchase # 5493SC

5493SC 10 µg (With Carrier) $199.00
5493SF 10 µg (Carrier Free) $199.00
5493LC 50 µg (With Carrier) $599.00
5493LF 50 µg (Carrier Free) $599.00
$ 0. 00

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Source / Purification

Recombinant human Epigen (hEpigen) Ala24-Ala104 (Accession #Q6UW88-1) was produced in E. coli at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEpigen. All lots are greater than 98% pure.


Molecular Formula:

Recombinant hEpigen does not have a Met on the amino terminus and has a calculated MW of 9,087. DTT-reduced and non-reduced protein migrate as 8 kDa polypeptides with non-reduced protein having slightly greater mobility due to intramolecular cystines. The expected amino-terminal AVTVT of recombinant hEpigen was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant hEpigen was determined in a MCF-10A cell proliferation assay. The ED50 of each lot is between 2-30 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hEpigen.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hEpigen. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Epigen was identified as an EGF family member by amino acid sequence homology (1). The sequence was obtained from mouse keratinocyte mRNA. Epigen is expressed in normal human mammary tissue and in some prostate and breast carcinomas (2). Epigen binding to known EGF receptors is with lower affinity when compared to EGF. However, Epigen appears to be superior in inducing cell proliferation (2). Epigen induces responses in MDA-MB-468 breast carcinoma cells. Epigen also has effects on prostate cells and vascular endothelial cells (2). Epigen binds to ErbB1/Her1 (2). Binding causes receptor homodimerization and heterodimerization with other Erb/Her receptors (2).


1.  Strachan, L. et al. (2001) J Biol Chem 276, 18265-71.

2.  Kochupurakkal, B.S. et al. (2005) J Biol Chem 280, 8503-12.


Entrez-Gene Id 255324
Swiss-Prot Acc. Q6UW88


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.