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6203S 300 µl (3 nmol) $249.00
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Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® c-Jun siRNA I (+) or SignalSilence® c-Jun siRNA II #6204 (+), using c-Jun (60A8) Rabbit mAb #9165 and α-Tubulin (11H10) Rabbit mAb #2125. The c-Jun (60A8) Rabbit mAb confirms silencing of c-Jun expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of c-Jun siRNA.

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Custom Ordering Details: This item is packaged to order. Please allow two to three days for your order to be processed and shipped.

Product Usage Information

CST recommends transfection with 100 nM c-Jun siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® c-Jun siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit c-Jun expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines, and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12).


1.  Jochum, W. et al. (2001) Oncogene 20, 2401-12.

2.  Davis, R.J. (2000) Cell 103, 239-52.

3.  Hilberg, F. et al. (1993) Nature 365, 179-81.

4.  Raivich, G. et al. (2004) Neuron 43, 57-67.

5.  Behrens, A. et al. (2002) EMBO J 21, 1782-90.

6.  Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.

7.  Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.

8.  Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.

9.  Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.

10.  Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.

11.  Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.

12.  Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.


Entrez-Gene Id 3725
Swiss-Prot Acc. P05412


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.