Upstream / Downstream

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To Purchase # 6236S

6236S 300 µl (3 nmol) $249.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY
H

Western blot analysis of extracts from MCF 10A cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Toll-like Receptor 3 siRNA I (+), using Toll-like Receptor 3 (D10F10) Rabbit mAb #6961 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Toll-like Receptor 3 (D10F10) Rabbit mAb confirms silencing of

Toll-like Receptor 3 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Learn more about how we got this image
Image

Product Usage Information

CST recommends transfection with 100 nM Toll-like Receptor 3 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® Toll-like Receptor 3 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Toll-like Receptor 3 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.


1.  Akira, S. (2003) J Biol Chem 278, 38105-8.

2.  Beutler, B. (2004) Nature 430, 257-63.

3.  Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.

4.  Medzhitov, R. et al. (1997) Nature 388, 394-7.

5.  Takeuchi, O. et al. (1999) Immunity 11, 443-51.

6.  Alexopoulou, L. et al. (2001) Nature 413, 732-8.

7.  Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.

8.  Horng, T. et al. (2001) Nat Immunol 2, 835-41.

9.  Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.

10.  Muzio, M. et al. (1997) Science 278, 1612-5.

11.  Wesche, H. et al. (1997) Immunity 7, 837-47.

12.  Suzuki, N. et al. (2002) Nature 416, 750-6.

13.  Irie, T. et al. (2000) FEBS Lett 467, 160-4.

14.  Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.


Entrez-Gene Id 7098
Swiss-Prot Acc. O15455


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.