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PathScan® Sandwich ELISA Lysis Buffer (1X) #7018
Gallery: PathScan® Sandwich ELISA Lysis Buffer (1X) #7018
PathScan® Sandwich ELISA Lysis buffer is used to create cell extract for ELISA applications.
1. If buffer will be used regularly, it is recommended that the 1X buffer be kept at 4ºC for 1-2 weeks. For longer periods of time, buffer should be stored at -20ºC. Aliquotting of 1X buffer is recommended to avoid freeze thaw cycles.
2. Thaw 1X buffer at 24-30ºC, mixing end-over-end.
3. Chill 1X buffer on ice and add PMSF just prior to use.Solutions and Reagents:
1X Lysis Buffer: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium Pyrophosphate, 25 mM Sodium Fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin
Note: Cell Signaling Technology recommends adding 1 mM PMSF immediately before use.Storage: Store at -20°C. For short term storage (1-2 weeks), Cell Lysis Buffer can be stored at 4°C.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. PathScan® is a trademark of Cell Signaling Technology, Inc.