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Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at

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Product Includes Volume Cap Color
Total Histone H3 Capture Ab (100X) 0.4 ml Pink
Acetylated-Lysine Detection Ab (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody 0.04 ml Yellow

Product Description

CST's PathScan® Acetylated Histone H3 Sandwich ELISA Antibody Pair is being offered as an alternative to our PathScan® Acetylated Histone H3 Sandwich ELISA Kit #7232. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The Histone Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by an Acetylated-Lysine Detection Antibody and HRP-conjugated Anti-Mouse IgG antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of Acetylated Histone H3 protein.

*Antibodies in this kit are custom formulations specific to the kit

Product Usage Information

Antibody pairs have been optimized using recommended buffers, reagents, plates and the included protocol. Solutions should be made fresh daily.

Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

1.  Dai J et al. (2005) Genes Dev 19, 472–88

2.  Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.

3.  Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.

4.  Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.

5.  Cheung, P. et al. (2000) Cell 103, 263-71.

6.  Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.

7.  Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.

8.  Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.

9.  Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.

10.  Goto, H. et al. (1999) J Biol Chem 274, 25543-9.

11.  Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.

Entrez-Gene Id 8350
Swiss-Prot Acc. P68431

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

PathScan® Acetylated Histone H3 Sandwich ELISA Antibody Pair