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Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

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REACTIVITY
Product Includes Volume Cap Color
p38 alpha MAPK Capture Ab (100X) 0.4 ml Pink
P-p38 MAPK(T180/Y182) Detection Ab (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody 0.04 ml Yellow

Product Description

CST's PathScan® Phospho-p38α MAPK (Thr180/Tyr182) Sandwich ELISA Antibody Pair is offered as an alternative to our PathScan® Phospho-p38α MAPK (Thr180/Tyr182) Sandwich ELISA Kit #7140. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. p38α MAPK Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by a Phospho-p38α MAPK (Thr180/Tyr182) Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of phospho-p38α MAPK (Thr180/Tyr182) protein.

*Antibodies in this kit are custom formulations specific to the kit.


Product Usage Information

Antibody pairs have been optimized using recommended buffers, reagents, plates and the included protocol. Solutions should be made fresh daily.


Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).

SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).


1.  Han, J. et al. (1994) Science 265, 808-11.

2.  Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.

3.  Rouse, J. et al. (1994) Cell 78, 1027-1037.

4.  Lee, J.C. et al. (1994) Nature 372, 739-46.

5.  Freshney, N.W. et al. (1994) Cell 78, 1039-49.

6.  Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.

7.  Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.

8.  Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

9.  Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.

10.  Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.


Entrez-Gene Id 1432
Swiss-Prot Acc. Q16539


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PathScan® is a trademark of Cell Signaling Technology, Inc.