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PhosphoPlus® p38 MAPK (Thr180/Tyr182) In-Cell Duet (ICW Compatible) #7257
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Analysis of A431 cells exposed to anisomycin (25 μg/mL) for varying lengths of time (5 – 60 min). The phosphorylation status of p38, as well as the total protein expression level, was measured simultaneously using the PhosphoPlus® p38 MAPK (Thr180/Tyr182) In-Cell Duet (ICW Compatible) #7257. Stimulation with anisomycin for short (15 min) and long (60 min) periods of time results in a 2-3 fold increase in phosphorylation of p38 as compared to the untreated control, while total p38 protein levels remained unchanged and were used to normalize the data. Data and images were generated using the LI-COR® Biosciences Odyssey® Infrared Imaging System.Learn more about how we got this image
Gallery: PhosphoPlus® p38 MAPK (Thr180/Tyr182) In-Cell Duet (ICW Compatible) #7257
PhosphoPlus® p38 MAPK (Thr180/Tyr182) In-Cell Duet from Cell Signaling Technology (CST) provides an easy method to assess protein activation status using a multi-well plate scanner with near infrared detection capabilities, such as the LI-COR® Biosciences Odyssey® Infrared Imaging System. This kit contains a pre-optimized activation-state and total protein antibody cocktail, selected based on superior performance. Phosphorylated and total protein are detected simultaneously in the same well, allowing levels of phosphorylated protein to be normalized to total protein. A near infrared detection cocktail is also included.
Phospho-p38 MAPK (Thr180/Tyr182) detects endogenous levels of p38 MAPK only when phosphorylated at Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Total p38α MAPK detects endogenous levels of total p38α MAPK. This antibody does not cross-react with either JNK/SAPK, p42/44 MAPK, or other isoforms of p38.
Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK or recombinant p38 MAPK protein.
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as ERK6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. PhosphoPlus® is a trademark of Cell Signaling Technology, Inc. LI-COR® is a registered trademark of LI-COR, Inc. Odyssey® is a registered trademark of LI-COR, Inc.