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SignalKine™ Human TNF-α Sandwich ELISA Kit #7289
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Figure 1. Time course of TNF-α secretion from THP-1 cells after LPS treatment. THP-1 cells were treated with TPA #4174 (16 nM, 18 hr) to induce differentiation. Addition of LPS (1 μg/ml) results in an increase of hTNF-α levels in the supernatant collected after 3, 6, and 24 hours compared with untreated cells using the SignalKine™ Human TNFα Sandwich ELISA Kit. Inset: Low picogram levels of hTNF-α in the untreated sample are detected by the SignalKine™ Human TNFα Sandwich ELISA Kit at 24 hours.Learn more about how we got this image
Gallery: SignalKine™ Human TNF-α Sandwich ELISA Kit #7289
- Figure 1. Time course of TNF-α secretion from THP-1 cells after LPS treatment. THP-1 cells were treated with TPA #4174 (16 nM, 18 hr) to induce differentiation. Addition of LPS (1 μg/ml) results in an increase of hTNF-α levels in the supernatant collected after 3, 6, and 24 hours compared with untreated cells using the SignalKine™ Human TNFα Sandwich ELISA Kit. Inset: Low picogram levels of hTNF-α in the untreated sample are detected by the SignalKine™ Human TNFα Sandwich ELISA Kit at 24 hours.
|Product Includes||Volume||Solution Color|
|TNF-α Rabbit mAb Coated Microwells||96 tests|
|Human TNF-α Standard (Lyophilized)||1 vial|
|SignalKine™ Assay Diluent A01||1.5 ml||Colorless|
|SignalKine™ Sample Diluent S01||25 ml||Brown|
|TNF-α Detection Rabbit mAb (Lyophilized) 100X||1 vial|
|Detection Antibody Diluent||6 ml||Green|
|HRP-linked Streptavidin (Lyophilized) 100X||1 vial|
|HRP Diluent||6 ml||Red|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|TMB Substrate 7004||6 ml||Colorless|
|STOP Solution 7002||6 ml||Colorless|
|Sealing Tape||2 sheets|
SignalKine™ Human TNF-α Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human TNF-α (hTNF-α) in multiple matrices. Unknown samples being tested for hTNF-α and hTNF-α standards are added to low volume microwells, where the hTNF-α is captured by the coated hTNF-α Rabbit mAb. Following a washing step, a biotinylated hTNF-α Detection Rabbit mAb is added to detect the captured hTNF-α. HRP-linked Streptavidin is then used for detection of the biotinylated hTNF-α Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hTNF-α in the sample.
SignalKine™ Human TNF-α Sandwich ELISA Kit detects hTNF-α in multiple matrices that can be quantified by generating a standard curve with the recombinant hTNF-α protein standard provided. The hTNF-α standard range is from 15.6 to 1000 pg/ml. Samples containing higher levels of hTNF-α can be diluted to fit into the standards range.
TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.