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REACTIVITY
H
Product Includes Volume Solution Color
TNF-α Rabbit mAb Coated Microwells 96 tests
Human TNF-α Standard (Lyophilized) 1 vial
SignalKine™ Assay Diluent A01 1.5 ml Colorless
SignalKine™ Sample Diluent S01 25 ml Brown
TNF-α Detection Rabbit mAb (Lyophilized) 100X 1 vial
Detection Antibody Diluent 6 ml Green
HRP-linked Streptavidin (Lyophilized) 100X 1 vial
HRP Diluent 6 ml Red
ELISA Wash Buffer (20X) 25 ml Colorless
TMB Substrate 7004 6 ml Colorless
STOP Solution 7002 6 ml Colorless
Sealing Tape 2 sheets

Order Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

Product Description

SignalKine™ Human TNF-α Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human TNF-α (hTNF-α) in multiple matrices. Unknown samples being tested for hTNF-α and hTNF-α standards are added to low volume microwells, where the hTNF-α is captured by the coated hTNF-α Rabbit mAb. Following a washing step, a biotinylated hTNF-α Detection Rabbit mAb is added to detect the captured hTNF-α. HRP-linked Streptavidin is then used for detection of the biotinylated hTNF-α Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hTNF-α in the sample.

SignalKine™ Human TNF-α Sandwich ELISA Kit detects hTNF-α in multiple matrices that can be quantified by generating a standard curve with the recombinant hTNF-α protein standard provided. The hTNF-α standard range is from 15.6 to 1000 pg/ml. Samples containing higher levels of hTNF-α can be diluted to fit into the standards range.


Specificity / Sensitivity


Species Reactivity: Human

TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).


1.  Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.

2.  Hehlgans, T. and Pfeffer, K. (2005) Immunology 115, 1-20.

3.  Lin, P.L. et al. (2007) J Investig Dermatol Symp Proc 12, 22-5.


Entrez-Gene Id 7124
Swiss-Prot Acc. P01375


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.