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REACTIVITY SENSITIVITY MW (kDa) SOURCE
4 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

β-Amyloid (1-42 Specific) Antibody recognizes the Aβ-42 isoform of the β-amyloid peptides.


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Source / Purification

Polycolonal antibodies are produced by immunizing rabbits with a synthetic peptide corresponding to residues at the carboxy terminus of human β-amyloid (1-42) peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).


1.  Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.

2.  Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.

3.  Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.

4.  Suzuki, T. et al. (1994) EMBO J 13, 1114-22.

5.  Ando, K. et al. (1999) J Neurosci 19, 4421-7.

6.  Iijima, K. et al. (2000) J Neurochem 75, 1085-91.


Entrez-Gene Id 351
Swiss-Prot Acc. P05067


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