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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of Sox2 protein.


Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog, Horse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Sox2 (D6D9) XP® Rabbit mAb #3579.


Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal (1). Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state (2,3). Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development (4,5). siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (6). Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state (7,8). Additional evidence demonstrates that Sox2 is also present in adult multipotent progenitors that give rise to some adult epithelial tissues, including several glands, the glandular stomach, testes, and cervix. Sox2 is thought to regulate target gene expression important for survival and regeneration of these tissues (9).


1.  Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.

2.  Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.

3.  Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.

4.  Boyer, L.A. et al. (2005) Cell 122, 947-56.

5.  Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.

6.  Matin, M.M. et al. (2004) Stem Cells 22, 659-68.

7.  Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.

8.  Okita, K. et al. (2007) Nature 448, 313-7.

9.  Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.


Entrez-Gene Id 6657
Swiss-Prot Acc. P48431


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.