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Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of Sox2 protein.

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog, Horse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Sox2 (D6D9) XP® Rabbit mAb #3579.

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).

1.  Nichols, J. et al. (1998) Cell 95, 379-391.

2.  Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.

3.  Boyer, L.A. et al. (2005) Cell 122, 947-56.

4.  Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.

5.  Matin, M.M. et al. (2004) Stem Cells 22, 659-668.

6.  Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.

7.  Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.

8.  Rodda, D.J. et al. (2005) J Biol Chem 280, 24731-7.

Entrez-Gene Id 6657
Swiss-Prot Acc. P48431

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.