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To Purchase # 7720S
|7720S||1050 µl (70 lanes)||$89.00|
|7720L||5250 µl (350 lanes)||$349.00|
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Prestained Protein Marker, Broad Range (Premixed Format) #7720
Gallery: Prestained Protein Marker, Broad Range (Premixed Format) #7720
Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 7 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below.
(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.
(b) Heat the Marker to 95-100ºC for 3-5 minutes.
(c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells.Solutions and Reagents:
Contents 0.1–0.2 mg/ml of each protein
Note: Prepare solutions with Milli-Q or equivalently purified water.
Wash Buffer (TBS/T): 20 mM Tris-HCl (pH 7.6), 137 mM NaCl and 0.1% Tween-20Storage: Store at -20°C.
|Protein||Source||Apparent MW (Da)|
|CBD-Mxe Intein-2CBD||E. coli||46,000|
|CBD-Mxe Intein||E. coli||30,000|
|Lysozyme||chicken egg white||17,000|
- chitin binding domain
- maltose-binding protein.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. This product is produced at New England Biolabs.