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REACTIVITY
Product Includes Volume Solution Color
SQSTM1/p62 Rabbit Antibody coated microwells 96 tests
SQSTM1/p62 Mouse Detection mAb 1 Ea Green (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) 1 Ea Red (Lyophilized)
Detection Antibody Diluent 11 ml Green
HRP Diluent 11 ml Red
TMB Substrate 7004 11 ml Colorless
STOP Solution 7002 11 ml Colorless
Sealing Tape 2 sheets
ELISA Wash Buffer (20X) 25 ml Colorless
ELISA Sample Diluent 25 ml Blue
Cell Lysis Buffer (10X) 9803 15 ml Yellowish

Product Description

The PathScan® Total SQSTM1/p62 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total SQSTM1/p62 protein. An SQSTM1/p62 Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, SQSTM1/p62 protein is captured by the coated antibody. Following extensive washing, SQSTM1/p62 Mouse Detection Antibody is added to detect the captured SQSTM1/p62 protein. Anti-Mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of total SQSTM1/p62 protein.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

PathScan® Total SQSTM1/p62 Sandwich ELISA Kit detects endogenous levels of SQSTM1/p62 protein in human cells as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Species Reactivity: Human, Mouse, Rat, Monkey, Mink

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity.


1.  Kirkin, V. et al. (2009) Mol Cell 34, 259-69.

2.  Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9.

3.  Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23.

4.  Bjørkøy, G. et al. (2006) Autophagy 2, 138-9.

5.  Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5.

6.  Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80.

7.  Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6.

8.  Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7.

9.  Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9.

10.  Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14.

11.  Komatsu, M. et al. (2007) Cell 131, 1149-63.

12.  Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45.


Entrez-Gene Id 115201
Swiss-Prot Acc. Q8WYN0


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.