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Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

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REACTIVITY
Product Includes Volume Cap Color
p53 Capture Ab (100X) 0.4 ml Pink
Acetylated-Lysine Detection Ab (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody 0.04 ml Yellow

Product Description

CST's PathScan® Acetylated p53 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Acetylated p53 Sandwich ELISA Kit #7236. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The p53 Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by an Acetylated-Lysine Detection Antibody and anti-Rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of acetylated p53 protein.

*Antibodies in this kit are custom formulations specific to the kit.


Product Usage Information

Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). p53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14). p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo (13,15). Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16). Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17). Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding (18). Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response (19).


1.  Chehab NH et al. (1999) Proc Natl Acad Sci U S A 96, 13777–82

2.  Levine, A.J. (1997) Cell 88, 323-31.

3.  Meek, D.W. (1994) Semin Cancer Biol 5, 203-10.

4.  Milczarek, G.J. et al. (1997) Life Sci 60, 1-11.

5.  Shieh, S.Y. et al. (1997) Cell 91, 325-34.

6.  Honda, R. et al. (1997) FEBS Lett 420, 25-7.

7.  Shieh, S.Y. et al. (1999) EMBO J 18, 1815-23.

8.  Hirao, A. et al. (2000) Science 287, 1824-7.

9.  Hao, M. et al. (1996) J Biol Chem 271, 29380-5.

10.  Tibbetts, R.S. et al. (1999) Genes Dev 13, 152-7.

11.  Lu, H. et al. (1997) Mol Cell Biol 17, 5923-34.

12.  Ullrich, S.J. et al. (1993) Proc Natl Acad Sci U S A 90, 5954-8.

13.  Kohn, K.W. (1999) Mol Biol Cell 10, 2703-34.

14.  Lohrum, M. and Scheidtmann, K.H. (1996) Oncogene 13, 2527-39.

15.  Sakaguchi, K. et al. (1998) Genes Dev 12, 2831-41.

16.  Knippschild, U. et al. (1997) Oncogene 15, 1727-36.

17.  Oda, K. et al. (2000) Cell 102, 849-62.

18.  Ito, A. et al. (2001) EMBO J 20, 1331-40.

19.  Solomon, J.M. et al. (2006) Mol Cell Biol 26, 28-38.


Entrez-Gene Id 7157
Swiss-Prot Acc. P04637


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.