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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Upf2 (D4A7) Rabbit mAb recognizes endogenous levels of total Upf2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu385 of human Upf2 protein.

Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).

1.  Leeds, P. et al. (1991) Genes Dev 5, 2303-14.

2.  Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.

3.  Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.

4.  Isken, O. et al. (2008) Cell 133, 314-27.

5.  Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.

Entrez-Gene Id 26019
Swiss-Prot Acc. Q9HAU5

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Upf2 (D4A7) Rabbit mAb