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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb 4858 1 x 100 µl
H M R Mk Mi Sc 32 Rabbit IgG
S6 Ribosomal Protein (5G10) Rabbit mAb 2217 1 x 100 µl
H M R Mk 32 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).


1.  Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.

2.  Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.

3.  Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.

4.  Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.

5.  Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.


Entrez-Gene Id 6194
Swiss-Prot Acc. P62753


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.