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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CHD3 Antibody 4241 40 µl
Western Blotting Immunoprecipitation
H M Mk 260 Rabbit 
CHD4 (D8B12) Rabbit mAb 11912 40 µl
Western Blotting Immunoprecipitation
H M R Mk 260 Rabbit IgG
HDAC1 (10E2) Mouse mAb 5356 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 62 Mouse IgG1
HDAC2 (3F3) Mouse mAb 5113 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 60 Mouse IgG1
MBD3 Antibody 3896 40 µl
Western Blotting
H M R Mk 28, 32, 34 Rabbit 
MTA1 (D40D1) XP® Rabbit mAb 5647 40 µl
Western Blotting Immunohistochemistry
H M R Mk 78-82 Rabbit IgG
RBAP46 (V415) Antibody 6882 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 48 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 

Product Description

NuRD Complex Antibody Sampler Kit offers an economical means of detecting each target protein that composes the nucleosome remodeling and deacetylation complex (NuRD). The kit contains enough primary antibody to perform four western blot experiments with each primary antibody.

Specificity / Sensitivity

All antibodies contained in this kit detect endogenous levels of their respective target protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

The transcriptional repressor NuRD (nucleosome remodeling and histone deacetylase) complex is composed of multiple subunits, which include CHD3 (also known as Mi2-α), CHD4 (also known as Mi2-β), HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (1-6). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression, and are also found together in the mSin3A and MeCP1 complexes (7-9). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target the NuRD complex to its histone substrates (10). MBD3, a member of the methyl-CpG-binding domain protein family, and the metastasis associated genes MTA1, MTA2, and MTA3 were all found to be integral components of the NuRD complex (2,3,7,11).

1.  Tong, J.K. et al. (1998) Nature 395, 917-21.

2.  Verreault, A. et al. (1998) Curr Biol 8, 96-108.

3.  Xue, Y. et al. (1998) Mol Cell 2, 851-61.

4.  Zhang, Y. et al. (1998) Cell 95, 279-89.

5.  Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.

6.  Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.

7.  Zhang, Y. et al. (1997) Cell 89, 357-64.

8.  Jones, P.L. et al. (1998) Nat Genet 19, 187-91.

9.  Fujita, N. et al. (2003) Cell 113, 207-19.

10.  Ng, H.H. et al. (1999) Nat Genet 23, 58-61.

11.  Wade, P.A. et al. (1999) Nat Genet 23, 62-6.

Entrez-Gene Id 1107 , 1108 , 3065 , 3066 , 53615 , 9112 , 5931
Swiss-Prot Acc. Q12873-3 , Q14839 , Q13547 , Q92769 , O95983 , Q13330 , Q16576

For Research Use Only. Not For Use In Diagnostic Procedures.
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