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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Tuberin/TSC2 (D93F12) XP® Rabbit mAb 4308 1 x 40 µl
H M R Hm Mk 200 Rabbit IgG
Phospho-Tuberin/TSC2 (Ser939) Antibody 3615 1 x 40 µl
H M 200 Rabbit 
Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb 3617 1 x 40 µl
H M R 200 Rabbit IgG
Phospho-Tuberin/TSC2 (Ser1387) Antibody 5584 1 x 40 µl
H M R Mk 200 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 1 x 100 µl
All Goat 

Product Description

The Phospho-TSC2 Antibody Sampler Kit provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform four western blot experiments per primary antibody.


Specificity / Sensitivity

Each antibody in the Phospho-TSC2 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members unless otherwise indicated. Phospho-Tuberin/TSC2 (Ser1387) Antibody detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody also cross-reacts with an unidentified 140 kDa protein.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human tuberin protein or a synthetic phosphopeptide corresponding to residues surrounding Thr1462 of human tuberin protein. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser939 or corresponding to residues surrounding Ser1387 of human tuberin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).


1.  Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.

2.  Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.

3.  Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.

4.  Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.

5.  Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.

6.  Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.

7.  Manning, B.D. et al. (2002) Mol Cell 10, 151-62.


Entrez-Gene Id 7249
Swiss-Prot Acc. P49815


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.