News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Product Description

The Cellular Localization Alexa Fluor® 647 Conjugated Antibody Sampler Kit provides an economical means for identification of cellular organelles without the need for a fluorescent secondary antibody. This kit includes enough primary antibody to perform at least 20 IF-IC tests per antibody.


Specificity / Sensitivity

β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform), but it does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms. COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total COX IV protein. PDI (C81H6) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PDI protein. β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-actin protein, residues surrounding Lys29 of human COX IV protein, the sequence of human PDI protein, or the amino terminus of human β-tubulin protein.

Knowledge of the subcellular location of a protein may reveal the potential role it plays in the cell. The subcellular location of a protein of interest may be confirmed by colocalization with one of the organelle-specific antibodies in this kit.

β-Actin and β-tubulin are ubiquitous eukaryotic proteins that form the major component of the cellular cytoskeleton (1,2). Cytochrome c oxidase (COX) IV is part of the hetero-oligomeric enzyme found in the inner mitochondrial membrane (3). Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of disulfide bonds in the endoplasmic reticulum (4).


1.  Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.

2.  Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

3.  Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.

4.  Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

8663
Cellular Localization Alexa Fluor® 647 Conjugated Antibody Sampler Kit