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REACTIVITY
H
Product Includes Volume Solution Color
hEGF Rabbit mAb Coated Microwells 96 tests
Human EGF Standard (Lyophilized) 1 vial
SignalKine™ Assay Diluent A01 1.5 ml Colorless
SignalKine™ Sample Diluent S02 25 ml Colorless
hEGF Detection Rabbit mAb (Biotinylated)(Lyophilized)100X 1 vial
Detection Antibody Diluent 6 ml Green
HRP-linked Streptavidin (Lyophilized) 100X 1 vial
HRP Diluent 6 ml Red
ELISA Wash Buffer (20X) 25 ml Colorless
TMB Substrate 7004 6 ml Colorless
STOP Solution 7002 6 ml Colorless
Sealing Tape 2 sheets

Order Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

Product Description

SignalKine™ Human EGF Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human EGF (hEGF) in multiple matrices. Unknown samples being tested for hEGF and hEGF Standards are added to low volume microwells, where the hEGF is captured by the coated hEGF Rabbit mAb. Following a washing step, a biotinylated hEGF Rabbit mAb is added to detect the captured hEGF. HRP-linked Streptavidin is then used for detection of the biotinylated hEGF Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of EGF in the sample.

SignalKine™ Human EGF Sandwich ELISA Kit detects hEGF in multiple matrices that be quantified by generating a standard curve with the recombinant hEGF protein standard provided. The hEGF standard range is from 3.9 to 250 pg/ml. Samples containing higher levels of hEGF can be diluted to fit into the standard range.


Specificity / Sensitivity


Species Reactivity: Human

Epidermal growth factor (EGF) is a small polypeptide hormone that has mitogenic properties, in vivo and in vitro, and affects the growth and/or differentiation of many cell types. EGF elicits biologic responses by binding to its cell surface receptor, which is a transmembrane glycoprotein containing a cytoplasmic protein tyrosine kinase (1,2). The binding of EGF to the EGF receptor promotes dimerization of the receptor, autophosphorylation, and activation of downstream signaling components (3). The integrated biological responses to EGF signaling are pleiotropic including mitogenesis, apoptosis, enhanced cell motility, protein secretion, differentiation, and dedifferentiation. In addition to being implicated in organ morphogenesis, maintenance, and repair, research studies have correlated upregulated EGF receptor signaling with progression to invasion and metastisis in a wide variety of tumors (4-6). Thus, investigators have identified EGF receptor and its downstream signaling molecules as potential targets for therapeutic interventions in wound repair and cancer (4-6).


1.  Wells, A. (1999) Int. J. Biochem. Cell. Biol. 31, 637-643.

2.  Boulougouris, P. and Elder, J. (2001) Anticancer Res. 21, 2769-2775.

3.  Schlessinger, J. (2002) Cell 110, 669-672.

4.  Sarries, C. et al. (2002) Pharmacogenomics 3, 763-780.

5.  Lorimer, I.A. (2002) Curr. Cancer Drug Targets 2, 91-102.

6.  Ghaneh, P. et al. (2002) J. Hepatobiliary Pancreat. Surg. 9, 1-11.


Entrez-Gene Id 1950
Swiss-Prot Acc. P01133


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.